Will my GPU lose x16 support if second PCIe slot is used for

hello everyone, I need a motherboard with at least 4 x16 pciei, i dont care about about pcie version I need to install four 10 gigabit pcie card

List of Motherboards with 4 PCI-Express x16 Slots: ASUS Pro WS TRX50-SAGE WIFI, GIGABYTE A620M C, GIGABYTE B650M C V2, ASUS Prime B760M-A AX, GIGABYTE B760M

pcie slot dimensions PCI Express expansion slots Desktop systems often offer a x16 slot intended for graphics cards and a x1 slot for PCI Express peripherals

pcie 4 card in pcie 3 slot PCIe x16 is your 16-lane PCI Express slot, or at least your largestlongest PCI Express slot It is the most high-bandwidth, high-performance expansion slot